Recyclable chaperone-conjugated magnetic beads for in vitro refolding of <Emphasis Type="Italic">Burkholderia cepacia</Emphasis> lipase |
| |
Authors: | Suhyun Jung Seongsoon Park |
| |
Institution: | (1) Department of Chemistry, Center for NanoBio Applied Technology, College of Natural Sciences, Sungshin Women’s University, Seoul, 136-742, South Korea;(2) Institute of Basic Sciences, Sungshin Women’s University, Seoul, 136-742, South Korea |
| |
Abstract: | Polymer-coated magnetic beads have become widely used in biological applications because of their facile recovery and easily
modifiable surface. Herein, we report the application of magnetic beads to in vitro refolding of B. cepacia lipase. Magnetic particles (Fe3O4) prepared by co-precipitation of Fe2+ and Fe3+ ions under basic conditions were subsequently coated with carboxylic acid-containing polystyrene by emulsion polymerization.
The polymer-coated magnetic beads were then conjugated with molecular chaperone proteins to assist with refolding. The chaperone-conjugated
magnetic beads efficiently refolded B. cepacia lipase and were easily reused. The beads showed comparable refolding activity to the soluble chaperone, and retained more
than 95% of their refolding activity after five cycles of refolding B. cepacia lipase. |
| |
Keywords: | Chaperone Lipase Magnetic beads Refolding |
本文献已被 PubMed SpringerLink 等数据库收录! |
|