Emergence of a mutagenic ochre suppressor mutation under lactose selection in appm mutant ofEscherichia coli harbouring the F′lacZU118 episome |
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Authors: | R Jayaraman |
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Institution: | (1) School of Biological Sciences, Madurai Kamaraj University, 625 021 Madurai, India |
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Abstract: | WhenEscherichia coli harbouring theppm (earlier calledadi) mutation and the F′lacZU118 episome is subjected to lactose selection in the presence of suboptimal concentrations of glycerol, Lac+ colonies emerge after 5–6 days. They are shown to harbour an ochre suppressor mutation at 15.15 min. Inactivation ofrecA results in approximately four-fold reduction in the response. In theppm — ochre suppressor double mutant background the leakiness of thelacZ allele carried by F′ CC105 is enhanced, suggesting misreading of a valine codon (GUG) as glutamic acid codon (GAG). This
is accompanied by reversion of thelacZ mutation tolacZ
+ (GTG → GAG). In LB medium both the leakiness and reversion are inhibited by streptomycin. Inactivation ofrecA did not affect leakiness but abolished reversion. These data are discussed in relation to the importance of allele leakiness
and restricted growth in stationary-phase (adaptive) mutagenesis. |
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Keywords: | ppm mutation stationary-phase mutagenesis adaptive mutagenesis ochre suppressor allele leakiness |
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