The control region of the F sex factor DNA transfer cistrons: Physical mapping by deletion analysis |
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Authors: | Russell Thompson and Mark Achtman |
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Institution: | (1) Abt. Trautner, Max-Planck-Institut für molekulare Genetik, Ihnestraße 63/73, D-1000 Berlin 33;(2) Present address: Institute of Virology, University of Glasgow, G11 5JR, Scotland |
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Abstract: | Summary A technique has been developed which allows the isolation of random deletions extending from unique restriction enzyme sites in plasmid DNA molecules. The method involves transformation of E. coli cells with linear plasmid DNAs generated by restriction enzyme cleavage. We have used this technique to map DNA transfer genes in the tra control region of F sex factor DNA. Deletions within EcoRI fragment f6 of F DNA have been isolated and used to assign physical locations to tra genes by a combination of genetic complementation tests, restriction enzyme analysis, DNA heteroduplexing and the analysis of the proteins synthesised in minicells and in vitro. Deletion analysis has also allowed the identification of the traK gene product. |
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