Automatic evaluation of antifungal volatile compounds on the basis of the dynamic growth process of a single hypha |
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| Authors: | Kibong Oh Hideaki Matsuoka Osao Sumita Kosuke Takatori Hiroshi Kurata |
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| Institution: | (1) Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16, Nakamachi, 184 Koganei, Tokyo, Japan;(2) Research and Development Division, Bio-Giken Inc., 1-5-8, Iwamotocho, 101 Chiyodaku, Tokyo, Japan;(3) Food & Drug Safety Center, Hatano Research Institute, 729-5, Ochiai, 257 Hadano, Kanagawa, Japan;(4) The Tokyo Kembikyoin Foundation, Kudanminami, 101 Chiyodaku, Tokyo, Japan |
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| Abstract: | An automatic analysing system was developed and employed for the evaluation of antifungal activity of volatile compounds in the gas phase. Aspergillus niger was inoculated on agar medium in the reaction vessel. The reaction vessel was incubated at 28° C for 24 h and then a volatile compound was introduced into the vessel either in a batch or flow manner. The antifungal activity of the respective compounds estimated in situ was expressed by the dynamic response parameters of a single hypha. All volatiles tested in the present system inhibited hyphal growth, except linalyl acetate: Limone and geraniol were the most inhibitory. In contrast, linalyl acetate promoted hyphal growth. By definition of the parameters, the fungicidal and fungistatic effects could be distinguished.
Correspondence to: H. Matsuoka |
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