In vitro excystment of the metacercaria of Plagiorchis species 1 (Trematoda, Plagiorchiidae) |
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Authors: | Dieter Bock |
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Affiliation: | Universität Ulm, Abt. Ökologie und Morphologie der Tiere (Biologie III), Oberer Eselsberg, D-7900, Ulm, Federal Republic of Germany |
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Abstract: | 1986. In vitro excystrnent of the metacercaria of Plagiorchis species 1 (Trematoda, Plagiorchiidae). International Journal for Parasitology 16: 641–645. An optimal hatching success of Plagiorchis species 1 metacercariae (100% excystment, active metacercariae, mean hatching speed 2–10 min, lowest variance of the mean speed) was observed after pretreatment in an HCl-pepsin solution at pH 2.0 and 42°C for 60–70 min, and incubation in a hatching medium at 42 °C and pH 7.3–8.0 with a bile salt (Nacholate), NaHCO3, and a reductant (cysteine with 100% N2). The minimum conditions for nearly 100% excystment with lower hatching speeds and higher variances were the presence of NaHCO3, an oxygen concentration reduced to about 3% in the gas phase, pH> 7.3 and a temperature near 30°C if Na-cholate was absent, or in the presence of the bile salt, a phosphate buffer at pH> 5.0 and room temperature only. Obviously some hatching factors acted interchangeably with compensation for missing stimuli by others. The effect of the bile salt was comparable with that of other surfactants. The metacercariae excysted in nonenzymatic media, which implies an active hatching mechanism. |
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Keywords: | Plagiorchis species 1 Trematoda Plagiorchiidae metacercaria hatching, host intestine, excystment stimuli |
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