EagI andNotI linking clones from human chromosomes 11 and Xp |
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| Authors: | Mark A. Pook Rekhaben Thakrar Bruce Pottinger Brian Harding David Porteous Veronica van Heningen John Cowell Carol Jones Sue Povey Kay E. Davies Rajesh V. Thakker |
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| Affiliation: | (1) MRC Molecular Endocrinology Group, Collier Building, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK Tel. +44-181-7403014; fax +44-181-7498341, GB;(2) MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH24 2XU, UK, GB;(3) Department of Neurosciences, Cleveland Clinic Foundation, Cleveland, Ohio 44193, USA, US;(4) Eleanor Roosevelt Institute of Cancer Research, 1899 Gaylor Street, Denver, Colorado, CO 80206, USA, US;(5) The Galton Laboratory, MRC Human Biochemical and Genetics Unit, University College London, Wolfson House, 4 Stephenson Way, London NW1 2HE, UK, GB;(6) Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK, GB |
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| Abstract: | EagI and NotI linking libraries were prepared in the lambda vector, EMBL5, from the mouse-human somatic cell hybrid 1W1LA4.9, which contains human chromosomes 11 and Xp as the only human component. Individual clones containing human DNA were isolated by their ability to hybridise with total human DNA and digested with SalI and EcoRI to identify the human insert size and single-copy fragments. The mean (± SD) insert sizes of the EagI and NotI clones were 18.3 ± 3.2 kb and 16.6 ± 3.6 kb, respectively. Regional localisation of 66 clones (52 EagI, 14 NotI) was achieved using a panel of 20 somatic cell hybrids that contained different overlapping deletions of chromosomes 11 or Xp. Thirty-nine clones (36 EagI, 3 NotI) were localised to chromosome 11; 17 of these were clustered in 11q13 and another nine were clustered in 11q14–q23.1. Twenty-seven clones (16 EagI, 11 NotI) were localised to Xp and 10 of these were clustered in Xp11. The 66 clones were assessed for seven different microsatellite repetitive sequences; restriction fragment length polymorphisms for five clones from 11q13 were also identified. These EagI and NotI clones, which supplement those previously mapped to chromosome 11 and Xp, should facilitate the generation of more detailed maps and the identification of genes that are associated with CpG-rich islands. Received: 27 December 1995 / Revised: 30 January 1996 |
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