Procoagulant activity of mouse transformed cells: Different expression in freshly isolated or cultured cells |
| |
Authors: | Curatolo Laura Alessio Maria Orazia Casali Bruno Falanga Anna Donati Maria Benedeita Semeraro Nicola |
| |
Affiliation: | (1) Istituto di Ricerche Farmacologiche “Mario Negri”, via Eritrea 62, 20157 Milano, Italy;(2) Istituto di Patologia Generale, Universita di Bari, Policlinico, P.zza G. Cesare, 70124 Bari, Italy;(3) Present address: Department of Oncology, Farmitalia-Carlo Erba, Nerviano, Italy;(4) Present address: Consorzio “mario Negri Sud”, Mario Negri Institute for Pharmacological Research, 66030 S. Maria Imbaro Chieti, Italy |
| |
Abstract: | Summary This study was originally designed to investigate wheter there is any correlation between the type of procoagulant activity (PCA) and the tumorigenicity of transformed cells. The data obtained are relevant to this question and to defining the differences in the expression of cellular activities depending on the in vitro system used. PCA was measured and characterized in normal, immortalized, and tumorigenic mouse fibroblasts. In all the lines studied the activity was of tissue factor type, as established with functional, enzymatic, and immunochemical criteria. However, the PCA of cells freshly isolated from the tumors induced by tumorigenic cell lines was of cancer procoagulant type, i.e. a cysteine protease with direct factor X activator activity. The same cells, when cultured in vitro, expressed again PCA of tissue-factor type. These results suggest that either a tumor-host interaction is required for the expression of cancer procoagulant or the latter activity, produced by tumor cells under in vitro conditions, is destroyed or inactivated during the culture period. Our findings caution against defining the procoagulant activity of tumors based on experiments on cultured cells. This work was partially supported by the Italian National Research Council and by the Italian Association for Cancer Research. |
| |
Keywords: | procoagulants proteases tissue factor cancer cells |
本文献已被 SpringerLink 等数据库收录! |
|