Preliminary crystallographic study of phospholipase A2 from the venom of Trimeresurus flavoviridis (habu snake) |
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| Authors: | Y Shinoda A Shouji T Yamane A Suzuki T Ashida H Kihara M Ohno |
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| Affiliation: | Department of Applied Chemistry, Faculty of Engineering, Nagoya University, Aichi. |
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| Abstract: | Crystallization and a preliminary crystallographic study of Trimeresurus flavoviridis (habu snake) phospholipase A2 (PLA2) were carried out. Although crystals were obtained from various solutions, crystals suitable for X-ray analysis could be obtained from polyethylene glycol solutions only when a repeated seeding technique was applied starting from twinned crystals. The crystal is monoclinic with space group P21, with a = 44.1, b = 55.7, c = 48.8 A, and beta = 92.4 degrees. An asymmetric unit contains a dimer consisting of two identical subunits made of 122 amino acids. The crystal reflects X-rays beyond 2.5 A. A Pt derivative gave a good isomorphous crystal. |
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