Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice |
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Authors: | S. Hittalmani A. Parco T. V. Mew R. S. Zeigler N. Huang |
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Affiliation: | (1) International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH |
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Abstract: | Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified. New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance to leaf blast in the Philippines and India indicated that combinations including Piz-5 have enhanced resistance than when it is present alone. The genes from the pyramided lines are at present being deployed into agronomically superior ricevarieties by marker-aided selection (MAS). Received: 20 June 1997 / Accepted: 14 September 1999 |
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Keywords: | RFLP markers Fine-mapping Blast resistance Pyramiding Marker-assisted selection |
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