Murine Ileocolic Bowel Resection with Primary Anastomosis |
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Authors: | Troy Perry Anna Borowiec Bryan Dicken Richard Fedorak Karen Madsen |
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Affiliation: | 1Department of Surgery, University of Alberta;2Department of Medicine, University of Alberta |
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Abstract: | Intestinal resections are frequently required for treatment of diseases involving the gastrointestinal tract, with Crohn’s disease and colon cancer being two common examples. Despite the frequency of these procedures, a significant knowledge gap remains in describing the inherent effects of intestinal resection on host physiology and disease pathophysiology. This article provides detailed instructions for an ileocolic resection with primary end-to-end anastomosis in mice, as well as essential aspects of peri-operative care to maximize post-operative success. When followed closely, this procedure yields a 95% long-term survival rate, no failure to thrive, and minimizes post-operative complications of bowel obstruction and anastomotic leak. The technical challenges of performing the procedure in mice are a barrier to its wide spread use in research. The skills described in this article can be acquired without previous surgical experience. Once mastered, the murine ileocolic resection procedure will provide a reproducible tool for studying the effects of intestinal resection in models of human disease. |
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Keywords: | Medicine Issue 92 Ileocolic resection anastomosis Crohn''s disease mouse models intestinal adaptation short bowel syndrome |
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