EFFECT OF TRITIATED THYMIDINE ON THE KINETICS AND VIABILITY OF 9L CELLS IN VITRO |
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| Authors: | M Wiezsaecker† T Hoshino S Kobayashi |
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| Institution: | Brain Tumor Research Center, Department of Neurological Surgery, School of Medicine, University of California, San Francisco, U.S.A. |
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| Abstract: | The colony-forming efficiency of 9L rat gliosarcoma cells was unaffected by treatment with 0.1 μCi/ml of 3H]TdR. However, when cells were treated with 1 or 10 μCi/ml of 3H]Tdr, cell growth was reduced and cell survival decreased. When monolayer 9L cells were treated with 1 μCi/ml of 3H]TdR for up to 72 hr, approximately 5% survived, which is closely related to the percentage of non-cycling cells in this system. When cells were treated with 10 μCi/ml of 3H]TdR for 72 hr, less survival was observed. the additional cell kill observed may be induced by 3H]TdR released from doomed cells into petri dishes during the incubation period of the colony-formation assay. |
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