Signal-on fluorescent sensor based on N-CQDs for the detection of glutathione in human serum and pharmaceutic preparation |
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| Authors: | Chengfei Zhao Kangying Wang Qihong Cai Haijian Tu Linghong Pan Liumin Yu |
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| Affiliation: | 1. Department of Pharmacy, Pharmacy and Medical Technology School, Putian University, Putian, China;2. Key Laboratory for Modern Pharmaceutical Analysis and Laboratory Medicine of Fujian province, Pharmacy and Medical Technology School, Putian University, Putian, China;3. Department of Clinical Laboratory, Affiliated Hospital (Group) of Putian University, Putian, China |
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| Abstract: | Nitrogen-doped carbon quantum dots (N-CQDs) with citric acid and ethylenediamine as raw materials were synthesized by an efficient one-step strategy. The N-CQDs showed a special property that the fluorescence was quenched by Fe3+. The quenched fluorescence of N-CQDs could be recovered by glutathione (GSH). Therefore, a “signal-on” fluorescent sensor was developed to detect GSH. The fluorescent sensor could favorably avoid the interference of ascorbic acid, dopamine, glucose, oxidized glutathione, and other amino acids in the detecting process of GSH. The proposed sensor showed a great feature that GSH can be accurately detected in the range from 0.001 to 0.1?mol/L and can be applied to detect GSH in the human serum. Therefore, the proposed method has a promising application for monitoring the blood drug concentration of GSH in clinical studies. |
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| Keywords: | Carbon nanomaterials fluorescent sensor glutathione human serum nitrogen-doped carbon quantum dots |
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