Batch and fed-batch bioreactor studies for the enhanced production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428 |
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Authors: | Sanjay Kumar Ashish A Prabhu Kannan Pakshirajan |
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Institution: | Biochemical Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, India |
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Abstract: | The effect of dissolved oxygen (DO) level and pH (controlled/uncontrolled) was first studied to enhance the production of novel glutaminase-free L-asparaginase by Pectobacterium carotovorum MTCC 1428 in a batch bioreactor. The optimum level of DO was found to be 20%. The production of L-asparaginase was found to be maximum when pH of the medium was maintained at 8.5 after 12?h of fermentation. Under these conditions, P. carotovorum produced 17.97?U/mL of L-asparaginase corresponding to the productivity of 1497.50?U/L/h. The production of L-asparaginase was studied in fed-batch bioreactor by feeding L-asparagine (essential substrate for production) and/or glucose (carbon source for growth) at the end of the reaction period of 12?h. The initial medium containing both L-asparagine and glucose in the batch mode and L-asparagine in the feeding stream was found to be the best combination for enhanced production of glutaminase-free L-asparaginase. Under this condition, the L-asparaginase production was increased to 38.8?U/mL, which corresponded to a productivity of 1615.8?U/L/h. The production and productivity were increased by 115.8% and 7.9%, respectively, both of which are higher than those obtained in the batch bioreactor experiments. |
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Keywords: | Antileukemic enzyme bioreactor fed-batch fermentation L-asparaginase" target="_blank">L-asparaginase Pectobacterium carotovorum |
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