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Comparison between the univariate and multivariate analysis on the partial characterization of the endoglucanase produced in the solid state fermentation by Aspergillus oryzae ATCC 10124
Authors:Aila Riany de Brito  Nadabe dos Santos Reis  Tatielle Pereira Silva  Renata Cristina Ferreira Bonomo  Ana Paula Trovatti Uetanabaro  Sandra Aparecida de Assis
Institution:1. Pos-Graduation Program in Food Engineering, Department of Basic and Instrumental Studies, State University of Southwest Bahia, Ilhéus, Bahia, Brazil;2. Pos-Graduation Program in Chemistry, Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, Bahia, Brazil;3. Department of Animal and Rural Technology, State University of Southwest Bahia, Ilhéus, Bahia, Brazil;4. Department of Biological Sciences, State University of Santa Cruz, Ilhéus, Bahia, Brazil;5. Department of Health, State University of Feira de Santana, Ilhéus, Bahia, Brazil
Abstract:Endoglucanase production by Aspergillus oryzae ATCC 10124 cultivated in rice husks or peanut shells was optimized by experimental design as a function of humidity, time, and temperature. The optimum temperature for the endoglucanase activity was estimated by a univariate analysis (one factor at the time) as 50°C (rice husks) and 60°C (peanut shells), however, by a multivariate analysis (synergism of factors), it was determined a different temperature (56°C) for endoglucanase from peanut shells. For the optimum pH, values determined by univariate and multivariate analysis were 5 and 5.2 (rice husk) and 5 and 7.6 (peanut shells). In addition, the best half-lives were observed at 50°C as 22.8?hr (rice husks) and 7.3?hr (peanut shells), also, 80% of residual activities was obtained between 30 and 50°C for both substrates, and the pH stability was improved at 5–7 (rice hulls) and 6–9 (peanut shells). Both endoglucanases obtained presented different characteristics as a result of the versatility of fungi in different substrates.
Keywords:Box–Behnken design  filamentous fungi  food waste  peanut shells  rice hulls
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