Fast activated charcoal prepurification of Fusarium solani β-glucosidase for an efficient oleuropein bioconversion |
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Authors: | Manel Boudabbous Walid Saibi Zouhaier Bouallagui Mosbeh Dardouri Sami Sayadi Hafedh Belghith |
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Institution: | 1. Laboratory of Molecular Biotechnology of Eucaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia;2. Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia;3. Environmental Bioprocesses Laboratory, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia |
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Abstract: | Fungal β-glucosidases were extensively studied regarding their various potential biotechnology applications. Here, we report the selection of Fusarium solani strain producing high yield of β-glucosidase activity. The effect of some factors on β-glucosidase production was studied including: Initial pH, medium composition, concentration of carbon and nitrogen sources, and particle size of raw substrates. The optimal enzyme production was obtained with 4?units of pH. The highest β-glucosidase activity was produced on 4% wheat bran (WB) as raw carbon sources, reaching 5?U/mL. A positive correlation between WB particle size and the β-glucosidase production level was settled. The last one was enhanced to 13.60?U/mL in the presence of 0.5% (w/v) of ammonium sulfate. Interestingly, the activated charcoal was used as an inexpensive reagent enabling a rapid and efficient purification prior step that improved the enzyme-specific activity. Eventually, F. solani β-glucosidase acts efficiently during the bioconversion process of oleuropein. Indeed, 82.5% of oleuropein was deglycosylated after 1?hr at 40°C. Altogether, our data showed that the β-glucosidase of F. solani has a potential application to convert oleuropein to ameliorate food quality. |
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Keywords: | Activated charcoal bioconversion oleuropein prepurification wheat bran β-glucosidase |
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