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Production of nanocellulose in miniature-bioreactor: Optimization and characterization |
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| Authors: | Sepideh Khazeni Fatemeh Yazdian Zahra Beagom Mokhtari-Hosseini Bahman Ebrahimi-Hosseinzadeh Behnam Noorani |
| Institution: | 1. Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran;2. Department of Chemical Engineering, Faculty of Petroleum and Petrochemical Engineering, Hakim Sabzevari University, Sabzevar, Iran |
| Abstract: | Bacterial cellulose (BC) is a very fascinating microbial biopolymer which is mainly produced by Gluconacetobacter xylinum. Optimization of BC production by G. xylinum was performed based on scale-down studies in miniature-bioreactor and response surface methodology in which the optimum pH value (6.5) and shaking rate (50?rpm) were obtained. The static culture condition for BC production has newly been defined. Nanostructure of BC includes nanofibers up to (60?nm) and nanoporosity up to (265?nm) was observed by scanning electron microscopy. By Fourier transform infrared spectroscopy study, the most expected BC interaction is nucleophilic interaction. MTT assay showed high biocompatibility. Appropriate mechanical strength (0.37?MPa) and Young’s modulus (3.36?MPa) evinced BC scaffold utilization for skin tissue. The results indicate that BC sheets can be utilized in biomedical application and nanotechnology approaches. |
| Keywords: | Bacterial cellulose Gluconacetobacter xylinum miniature-bioreactor optimization static culture |