Application of aqueous biphasic systems as strategy to purify tannase from Aspergillus tamarii URM 7115 |
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| Authors: | Amanda Reges de Sena Flávio Manoel Barros Oliveira Tonny Cley Campos Leite Talita Camila Evaristo da Silva Nascimento Keila Aparecida Moreira |
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| Institution: | 1. Microbiology Laboratory, Federal Institute of Education, Science and Technology of Pernambuco, Barreiros, Brazil;2. Biotechnology Laboratory, Garanhuns Academic Unit, Federal Rural University of Pernambuco, Garanhuns, Brazil |
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| Abstract: | The aims of the current study are to assess the influence of polyethylene glycol (PEG) concentration, molar mass, pH, and citrate concentrations on aqueous biphasic systems based on 24 factorial designs, as well as to check their capacity to purify tannase secreted by Aspergillus tamarii URM 7115. Tannase was produced through submerged fermentation at 26°C for 67?h in Czapeck-Dox modified broth and added with yeast extract and tannic acid. The factorial design was followed to assess the influence of PEG molar mass (MPEG 600; 4,000 and 8,000?g/?mol), and PEG (CPEG 20.0; 22.0 and 24.0% w/w) and citrate concentrations (CCIT 15.0, 17.5, and 20.0%, w/w), as well as of pH (6.0, 7.0, and 8.0) on the response variables; moreover, partition coefficient (K), yield (Y), and purification factor (PF) were analyzed. The most suitable parameters to purify tannase secreted by A. tamarii URM 7115 through a biphasic system were 600 (g/mol) MPEG, 24% (w/w) CPEG, 15% (w/w) CCIT at pH 6.0 and they resulted in 6.33 enzyme partition, 131.25% yield, 19.80 purification factor and 195.08 selectivity. Tannase secreted by A. tamarii URM 7115 purified through aqueous biphasic systems composed of PEG/citrate can be used for industrial purposes, since it presents suitable purification factor and yield. |
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| Keywords: | Aspergillus tamarii Aqueous two-phase system downstream processing PEG/citrate tannin acyl hydrolase |
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