The use of PCR to isolate a putative ABC transporter from Saccharopolyspora erythraea |
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| Authors: | Mary P O'Neill E Anne Eady Alan Radford Simon Baumberg Jonathan H Cove |
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| Institution: | Institut für Molekulare Infektionsbiologie, Röntgenring 11, 97070 Würzburg, Germany; Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy |
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| Abstract: | Abstract The uropathogenic Escherichia coli strain J96 (04:K6) is able to produce four adherence factors P-fimbriae ( pap and prs ), F1C-fimbriae ( foc ) and Type 1-fimbriae ( fim )], two α-hemolysins ( hfy I and II) and the cytotoxic necrotizing factor type 1 ( cnf 1). Using phenotypic test systems and genotypic analysis, it has been shown that the mutant strain J96-M1 has lost the hly II, prs and cnf 1 genes. The three virulence associated determinants are linked on one particular region on the chromosome, which is termed 'pathogenicity island II' (Pai II). |
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| Keywords: | Pathogenicity islands Urovirulence Hemolysin P-fimbriae Escherichia coli Cytotoxic necrotizing factor 1 |
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