The effect of urea, gamma- and UV-irradiation on physico-chemical characteristics of native and immobilized inulinase |
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Authors: | Kovaleva T A Kozhokina O M Trofimova O D |
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Institution: | Voronezh State University, Russia. |
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Abstract: | The immobilization of inulinase by ionexchange AB-26 and AB-17-2P has been made by adsorbtion and glutaraldehyde methods. The effect of UV-radiation, carbamide and gamma-rays on the stability of native and immobilized enzyme has been investigated. The stability of inulinase in relation to denaturation agents has been shown to increase with the immobilization of ionexchange. The character of binding with the matrix affects greatly the stability of immobilized enzyme to physical factors. |
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