A frameshift at a mutational hotspot in the polyoma virus early region generates two new proteins that define T-antigen functional domains |
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Authors: | J B Wilson A Hayday S Courtneidge M Fried |
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Affiliation: | 1. State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, PR China;1. Power Systems Laboratory, Department of Electrical Engineering and Informatics, ETH Zurich, 8092 Zurich, Switzerland;2. Division of Electricity, Department of Engineering Science, Uppsala Universitet, Angströmlaboriet, Uppsala, Sweden |
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Abstract: | A frameshift mutation, arising from the deletion of any one of nine consecutive cytidines in the region of Py DNA encoding both the midregion of large T-Ag and the C-terminal region of middle T-Ag, yields unstable flat cell revertants that synthesize two novel viral proteins in which shuffling of the different domains of the Py T-Ags has occurred. The first protein (37 kd) is a hybrid containing the N-terminus of large T-Ag and the hydrophobic C-terminus of middle T-Ag. The latter domain is responsible for membrane association, even in the 37 kd hybrid protein. The second protein (43 kd), which contains the N-terminal 75% of middle T-Ag, has an associated protein kinase activity and forms a complex with c-src, but cannot induce a transformed phenotype. |
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