Deoxyribonuclease I binding masks the major tryptic cleavage sites on actin |
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Authors: | L D Burtnick K W Chan |
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Affiliation: | Department of Chemistry, University of British Columbia Vancouver, B.C., Canada V6T 1Y6 |
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Abstract: | The formation of a 1:1 molecular complex of deoxyribonuclease I with muscle G-actin protects both proteins against proteolytic attack by trypsin. After 1 hours of digestion, negligible proteolysis of the complex is observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, while G-actin alone is rapidly broken down to a trypsin resistant core. The binding of deoxyribonuclease I to actin apparently masks the major tryptic cleavage sites (arginine-62 and lysine-68) on the latter protein. |
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