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Immunoreactive peptides related to dynorphin B (=rimorphin) in the rat brain |
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| Authors: | Y.X. Zhu, V. H llt,H. Loh |
| Affiliation: | a Department of Pharmacology, School of Medicine University of California, San Francisco, San Francisco, CA 94143, USA b Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie Kraepelinstraße 2, D-8000, München 40, FRG |
| Abstract: | We have developed a radioimmunoassay for synthetic dynorphin B, a novel opioid tridecapeptide, which shares a common precursor molecule with dynorphin1–17 (=dynorphin A) and the neo-endorphins. The levels of immunoreactivity towards this peptide in rat brain and pituitary show a pattern quantitatively and qualitatively similar to those found for dynorphin A and -neo-endorphin in earlier studies. The antiserum used was highly specific with only dynorphin-32 and dynorphin B-29, both of which contain the dynorphin B sequence, showing substantial cross-reactivity. Gel filtration of whole rat brain extracts in combination with HPLC analysis provide strong evidence for the existence of these latter two peptides in rat brain. |
| Keywords: | Dynorphin B Dynorphin B-29 Dynorphin-32 |
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