Comparative binding of albumin and -glucuronidase by rat liver microsomes |
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Authors: | L Mameli R Gianetto |
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Institution: | Départment de Biochimie, Universitéde Montréal, Montréal, Canada |
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Abstract: | Rat liver microsomes, free of lysosomal β-glucuronidase, were subjected to sonication. Under the experimental conditions used, 95 % of the microsomal β-glucuronidase activity was solubilized while only 11 % of the albumin was released in the soluble fraction. The results indicate that microsomal β-glucuronidase is not contained in the cisternae of the microsomal vesicles but is attached to the membranes by bonds that are broken by sonication before the membranes are disrupted. |
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