Interaction of histone H1 with superhelical DNA conformational studies and influence of ionic strength |
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Authors: | M. Böttger C. -U. von Mickwitz S. Scherneck R. Lindigkeit |
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Affiliation: | (1) Zentralinstitut für Molekularbiologie der Akademie der Wissenschaften der DDR, DDR-1115 Berlin-Buch, GDR |
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Abstract: | The interaction of histone H1 with superhelical SV40 DNA at low ionic strength (0.02 M NaCl) results in the formation of DNP double-fibers and bundle-and cablelike twisted side-by-side associates of several of these double-fibers. On the basis of simple cylindrical or ellipsoidal models the sedimentation properties of these structures can be calculated in accordance with the experiment allowing a direct assignment of electron microscopical and hydrodynamic results. Sedimentation measurements in dependence on the ionic strength indicate a redistribution of H1 resulting in the formation of associates at 0.04 M NaCl and of aggregates at higher salt concentration. Double-fibers are present up to physiological salt concentrations. |
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