Regulation of acetylcholinesterase activity by retinoic acid in a human neuroblastoma cell line |
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Authors: | N Sidell C A Lucas G W Kreutzberg |
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Affiliation: | 1. Division of Surgical Oncology, UCLA School of Medicine, Los Angeles, CA 90024, USA;2. Department of Nerve Cell Biology, Max Planck Institute for Psychiatry, 8000 Munich 40, FRG |
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Abstract: | The ability of retinoic acid (RA) to modulate acetylcholinesterase (AChE) activity in a human neuroblastoma cell line (LN-N-5) was examined. The specific activity of AChE was significantly increased 3 days after exposure of LA-N-5 to RA and reached its maximum values after 9 or more days of culturing. Dose-response experiments demonstrated that large increases of AChE occurred at RA concentrations between 10(-7) and 10(-6) M with maximum AChE values detected at 10(-6)-10(-5) M. Increased AChE activity paralleled neurite outgrowth in LA-N-5 cultures. These findings demonstrate that RA can regulate specific AChE activity in human neuroblastoma cells in a manner consistent with neuronal maturation. |
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Keywords: | To whom offprint requests should be sent. Address: Division of Surgical Oncology 54-140 Center for Health Sciences UCLA School of Medicine Los Angeles CA 90024 USA. |
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