Construction of a recombinant yeast strain converting xylose and glucose to ethanol |
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| Authors: | Shen Tian Jinxin Zang Yaping Pan Jikai Liu Zhenhong Yuan Yongjie Yan Xiushan Yang |
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| Institution: | 1. College of Life Science,Capital Normal University,Beijing 100037,China
2. East China University of Science and Technology,Shanghai 200237,China |
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| Abstract: | Candida shehatae gene xyll and Pichia stipitis gene xyl2,encoding xylose reductase (XR) and xylitol dehydrogenase (XD) respectively,were amplified by PCR.The genes xyl1 and xyl2 were placed under the control of promoter GAL in vector pYES2 to construct the recombinant expression vector pYES2-PI2.Subsequently the vector pYES2-P12 was transformed into S.cerevisiae YS58 by LiAc to produce the recombinant yeast YS58-12.The alcoholic ferment indicated that the recombinant yeast YS58-12 could convert xylose to ethanol with the xylose consumption rate of 81.3%. |
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| Keywords: | recombinant Saccaromyces cerevisiae xyl1 xyl2 xylose glucose ethanol |
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