Direct regeneration of shoots from immature inflorescence cultures of turmeric |
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Authors: | Salvi Neeta D George Leela Eapen Susan |
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Institution: | (1) Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK |
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Abstract: | Microspore-derived embryo (MDE) induction was evaluated following the culture of wheat (Triticum aestivum L.) anthers in a basal medium with a range of supplements. Several supplements were evaluated, including a commercial bovine
haemoglobin solution (Erythrogen™), Ficoll and the co-polymer surfactant Pluronic F-68, but these did not enhance MDE induction. However, phenylacetic acid,
replacing both 2,4-dichlorophenoxyacetic acid and kinetin, increased MDE induction by a factor of 1.6 (p<0.05), producing 257±29 (mean±s.e., n=3) MDEs per 100 anthers cultured. Twenty-four plants regenerated from MDEs induced via anther culture were used for ploidy
determinations; 33% were sterile haploids, while the remainder were fertile.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | anther culture doubled haploids microspore-derived embryos phenylacetic acid Poaceae Triticum aestivum L cv Pavon |
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