Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora (Munro) McClure) |
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Authors: | H S Tsay C C Yeh J Y Hsu |
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Institution: | (1) Department of Agronomy, Taiwan Agricultural Research Institute, Taichung, Taiwan 41301, Republic of China |
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Abstract: | Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of response for callus induction. Prolonged culture of these embryogenic calli on the original medium or subculture to an auxin-free medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. Chromosome counts from root-tip cells of anther-derived plant indicated that they were haploid (N=36).Abbreviations N6
Chu et al. (1975)
- MS
Murashige and Skoog (1962)
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
-naphthaleneacetic acid
- BA
6-benzylaminopurine |
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Keywords: | |
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