Multi-fed batch culture integrated with three-stage light irradiation and multiple additions of copper ions for the hyperproduction of ganoderic acid and Ganoderma polysaccharides by the medicinal mushroom Ganoderma lucidum |
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| Institution: | 1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, Hubei, China;2. Key Laboratory of Microbial Metabolism (Ministry of Education), College of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. Bioengineering College, Chongqing University, Chongqing 400044, China;2. Research Center for Tobacco Bioengineering and Technology, Chongqing Science and Technology Commission, Yubei District, Chongqing 401147, China;1. Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi 214122, China;2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;3. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;1. College of Food Science and Engineering, Northwest A&F University, Yang ling, Shaanxi, 712100, China;2. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China;3. Department of Food Science, University of Massachusetts, Amherst, MA01003, USA;4. College of Life Science and Engineering, Shaanxi University of Science & Technology, Xi''an, Shaanxi, 710021, China;1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China;2. College of Life Science and Technology, Shanghai Jiao Tong University, Shanghai, China;3. School of Public Health, Wuhan University, Wuhan 430071, China;4. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;1. Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People''s Republic of China;2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People''s Republic of China;3. Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, People''s Republic of China;4. Tianjin Vocation Technology College of Bioengineering, Tianjin 300462, People''s Republic of China |
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| Abstract: | To further enhance the accumulation of the bioactive metabolite ganoderic acid (GA) by fermentation of the medicinal mushroom Ganoderma lucidum, a novel integrated strategy was developed by simultaneously adopting a strategy of multiple Cu2+ additions, three-stage light irradiation and multi-pulse feeding of carbon and nitrogen sources. Maximal GA content (i.e., 4.1 mg/100 mg DW) and production (i.e., 720.8 mg/L) were obtained using the novel integrated strategy. Not only the biomass but also the total GA production obtained in this work is the highest reported for a shaker flask culture of G. lucidum. This work is useful for the large-scale production of GA by G. lucidum fermentation. |
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