Mechanism study on NS81006-mediated methanolysis of triglyceride in oil/water biphasic system for biodiesel production |
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| Institution: | 1. Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China;2. Amoy – BUCT Industrial Bio-technovation Institute, Amoy 361022, PR China;3. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China;1. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China;2. Institute of Environment Resource and Soil Fertilizer, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China;3. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;1. Enzyme Biotechnology & Genetic Engineering Group, University of Ioannina, Department of Chemistry, Ioannina 45110, Greece;2. Food Biotechnology Group, University of Patras, Department of Chemistry, Patras 26500, Greece |
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| Abstract: | Compared to immobilized lipase, soluble lipase has the merits of lower cost and faster reaction rate, thus much attention has been paid to soluble lipase-mediated methanolysis for biodiesel (fatty acid methyl ester, FAME) production in recent years. Our previous study showed that soluble lipase NS81006 could effectively catalyze the methanolysis of soybean oil (triglyceride, TG) for FAME preparation in oil/water biphasic system. Study on the related mechanism of soluble lipase NS81006-mediated methanolysis of TG was carried out in this paper. Based on the analysis of substances change in the reaction process, mechanism model was hypothesized and the model parameters were simulated by Matlab. The simulated model was validated further. The results showed that in the reaction process of soluble lipase NS81006-mediated methanolysis of TG in oil/water biphasic system, TG proceeded three-step hydrolysis to generate FFA (free fatty acid), and then FFA transformed into FAME by esterification with methanol. During the whole process, FFA is mainly generated through the hydrolysis of TG and intermediate DG (diglyceride), while the hydrolysis of FAME could be ignored. |
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