A new approach for the immobilization of permeabilized brewer's yeast cells in a modified composite polyvinyl alcohol lens-shaped capsule containing montmorillonite and dimethyldioctadecylammonium bromide for use as a biocatalyst |
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| Affiliation: | 1. College of Pharmaceutical Science, Chongqing Medical University, #1, Medical College Road, Chongqing 400016, China;2. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China;3. College of Environmental and Biological Engineering, Chongqing University of Technology and Business, Chongqing 400067, China;3. Physics Division, Faculty of Science, Cairo University, Egypt;1. School of Mechanical and Chemical Engineering, The University of Western Australia, Crawley 6009, Australia;2. School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore 599489 |
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| Abstract: | Permeabilized brewer's yeast cells were immobilized in modified composite polyvinyl alcohol lens-shaped capsules containing montmorillonite (MONT) and dimethyldioctadecylammonium bromide (DDAB). The special properties of the capsules were then investigated. The results showed that the diffusion capability of hydrophobic molecules from bulk solution into the capsules was obviously improved by the introduction of 0.5% MONT and 0.05% DDAB into the 15% PVA matrix. More importantly, when ethyl 4-chloro-3-oxobutanoate (COBE) was reduced with the capsules as the biocatalyst, the yield and enantiomeric excess (ee) of the product reached 88% and 99.1%, respectively, within 8 h, which are significantly higher totals than those mediated by the unmodified PVA capsules under the same conditions. The intracellular alcohol dehydrogenase (ADH) in the capsules retained over 81% of its original activity after 1 month of storage and 88% of its initial activity after 20 cycles of reaction. |
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