Integrated enzymatic production of specific structured lipid and phytosterol ester compositions |
| |
| Institution: | 1. Department of Human Nutritional Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada;2. Canadian Centre for Agri-food Research in Health and Medicine, Winnipeg, MB R3T 2N2, Canada;3. International Nutritional Research Inc., Winnipeg, Canada;4. Department of Biochemistry, Memorial University of Newfoundland, St John''s, NL A1B 3X9, Canada;5. College of Veterinary Medicine, Hunan Agricultural University, Changsha City, Hunan Province 410128, China |
| |
| Abstract: | Mixtures of specific structured lipids and phytosterol esters, valuable food components, were synthesized by an enzymatic one-pot process in organic-solvent-free medium starting from a mixture of phytosterol, caprylic acid and sunflower oil. Nine biocatalysts, seven commercially available lipases and two air-dried solid state (SSF) fermentation preparations of Aspergillus oryzae NRRL 6270 (AoSSF) and Aspergillus sojae NRRL 6271 (AsSSF), were screened for lipase activity in the transesterification reactions of sunflower oil with caprylic acid and for sterol esterase activity in the direct esterification of phytosterols with free fatty acids. The best process variant using a sequence of sterol esterase (AoSSF)-catalyzed esterification reaction of the free fatty acids and phytosterols, followed by water removal in vacuum and lipase-catalyzed transesterification with immobilized lipase from Rhizomucor miehei (Lipozyme) resulted in 92.1% conversion to phytosterol esters and 44.1% conversion to triacylglycerols containing two caprylic esters. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
