Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production |
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Affiliation: | 1. Department of Biochemistry, Chemistry Institute, Federal University of Rio de Janeiro, 21945900 RJ, Brazil;2. Laboratory Toxinology, IOC, Oswaldo Cruz Foundation, 21040900 RJ, Brazil;3. Leopoldo Américo Miguez de Mello Research and Development Center, PETROBRAS, 21949900 RJ, Brazil |
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Abstract: | The PA1 strain of Pseudomonas aeruginosa isolated from oil waste produces rhamnolipid, a biodegradable surfactant with applications in several industrial and environmental fields. The metabolic pathway and genetic regulation of rhamnolipid production in P. aeruginosa are poorly understood. Herein, several proteins directly or indirectly related to rhamnolipid production and their genetic regulations were identified by comparative proteomic. We compared the proteome of P. aeruginosa PA1 after fermentation in two different conditions of carbon and nitrogen sources: condition A allowed rhamnolipid production and condition B prevented it. Protein extracts from cellular pellets were compared using 2D-PAGE stained with colloidal Coomassie followed by MALDI-TOF/TOF mass spectrometry. We identified 21 differentially expressed proteins, including those involved in secretion, quorum sensing, oxidative response and metabolism. |
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