Corn husk as a novel substrate for the production of rifamycin B by isolated Amycolatopsis sp. RSP 3 under SSF |
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| Institution: | 1. N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia;2. D.K. Zabolotny Insitute of Microbiology and Virology, National Academy of Sciences, ul. Zabolotnogo 154, 03143, Kiev, Ukraine;1. Department of Geoinformatics and Cartography, Institute of Geography and Regional Development, Faculty of Earth Science and Environmental Management, University of Wroc?aw, pl. Uniwersytecki 1, 50-137 Wroc?aw, Poland;2. Wroc?aw Centre for Networking and Supercomputing, Wroc?aw University of Technology, Wybrze?e Wyspiańskiego 27, 50-370 Wroc?aw, Poland;1. State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, China;2. School of Marine Science and Technology, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom;1. ARC DECRA Fellow, Centre for Offshore Foundation Systems (COFS), The University of Western Australia, 35 Stirling highway, Crawley, WA 6009, Australia;2. Centre for Offshore Foundation Systems, The University of Western Australia, Australia;1. College of Continuing Education, Shijiazhuang Tiedao University, Shijiazhuang 050043, China;2. School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China;3. Technique R&D Center, Haier Group, Qingdao 266199, China;4. Tianjin Long March Launch Vehicle Manufacturing Co., Ltd., Tianjin 300462, China |
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| Abstract: | Rifamycin B production by isolated Amycolatopsis sp. RSP 3 was investigated under solid state fermentation (SSF) using agro-industrial waste materials. Corn husk was the most suitable substrate/support material with 4-fold higher production than wheat bran and corn cobs. A two-level (conventional and statistical) methodology was used to optimize fermentation parameters belong to physiological (pH, temperature and aeration), nutritional (carbon and nitrogen sources) and microbial (inoculum level and incubation time). Conventional optimization significantly improved (450%) the rifamycin B production of which two-third was associated with carbon and nitrogen sources. Starch as carbon source showed negative impact. Statistical optimization of suggested potassium nitrate (at individual level), soya bean meal and barbital (at interactive level) were observed to be the most noticeable variables in the maximization of production. At optimized conditions, inorganic nitrogen source played vital role (>59%) compared to all other factors. Overall, more than 920% increase in rifamycin B production was achieved at optimized environment. |
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