Enhanced production of succinic acid by Actinobacillus succinogenes with reductive carbon source |
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| Institution: | 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, Jiangsu, PR China;2. Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, PR China;3. Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Republic of Korea;1. National Food Institute, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark;2. Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea;1. Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China;2. National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China;3. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;1. School of Marine Sciences and Biotechnology, Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities, Nanning, Guangxi 530008, China;2. National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China;3. Biology Institute, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China;1. School of Water, Energy and Environment, Cranfield University, Cranfield MK43 0AL, UK;2. Department of Chemical, Environmental and Materials Engineering, Universidad de Jaén, Campus Las Lagunillas, 23071 Jaén, Spain;3. Department of Pharmaceutical Sciences, Kumaun University, Bhimtal, Nainital 263136, Uttarakhand, India;4. Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram 695 019, Kerala, India;5. Department of Life Sciences, Central University of Tamil Nadu, Neelakudi, Thiruvarur, Tamil Nadu 610005, India;6. School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea |
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| Abstract: | Carbon sources with different oxidation states were used to investigate the possibility increasing the availability of NADH and the NADH/NAD+ ratio and to determine the effect of this manipulation on the distribution of metabolites in Actinobacillus succinogenes NJ113. The sugars glucose, sorbitol and gluconate were each used at an initial concentration of 40 g/L.The yield of succinic acid (0.75) and the ratio of succinic acid to acetic acid (5.06) were both higher for sorbitol than the values obtained with glucose (0.66 and 2.68, respectively). In contrast, with gluconate as the carbon source the yield of succinic acid was 0.54 and the ratio of succinic acid to acetic acid was only 1.70. This work showed that different levels of NADH availability and the NADH/NAD+ ratio can be achieved by using carbon sources that have different oxidation states.Highly reduced sorbitol was examined as a possible carbon substrate for maximizing the redox potential during the production of succinic acid. |
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