Biosurfactants production by immobilized cells of Bacillus subtilis ATCC 21332 and their recovery by pertraction |
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| Affiliation: | 1. Institute of Food Science and Biotechnology, Department of Bioprocess Engineering, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany;2. Institute of Food Chemistry, Department of Food Chemistry and Analytical Chemistry, University of Hohenheim, Garbenstrasse 28, 70599 Stuttgart, Germany;1. Egyptian Petroleum Research Institute (EPRI), Nasr-City, Cairo, Egypt;2. Department of Botany and Microbiology, Faculty of Science, Helwan University, Egypt;1. College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China;2. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China;3. School of Molecular and Biomedical Science, University of Adelaide, Adelaide 5005, SA, Australia;1. Department of Natural Sciences, FCEFQyN, National University of Río Cuarto, Ruta Nacional 36 Km 601, Córdoba, Argentina;2. Department of Molecular Biology, FCEFQyN, National University of Río Cuarto, Ruta Nacional 36 Km 601, Córdoba, Argentina;3. IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina |
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| Abstract: | Microbial production and isolation of biosurfactants was studied. The production of lipopeptides surfactin and fengycin was performed by free and immobilized aerobic cells of Bacillus subtilis ATCC 21332. After preliminary tests with 5 polymer materials, the particles of polypropylene foamed with powder activated carbon (PPch) were selected for lipopeptides production for their thermal and mechanical stability and for the high colonizing effect. To avoid foaming during biosurfactant production, biofilm grown on solid floating support was aerated by air injected over the surface of cultural medium. The synthesis of both lipopeptides and especially of the fengycin was greatly enhanced by the immobilization. The relationship between support wettability, colonization of the cells, and lipopeptide production was discussed. Extraction behaviour of the lipopeptides into alkanes was studied. The distribution ratio of surfactin was found to be higher than this of fengycin at the same conditions and the n-heptane was more efficient solvent for both lipopeptides. Kinetics of surfactin recovery from fermentation broth applying batch pertraction in a rotating discs contactor was studied. Lipopeptide was successfully extracted (more than 75% in the first hour) using n-heptane as liquid membrane and a 0.2 mol L−1 phosphate buffer solution (pH ∼ 7.3) as receiving solution. However, the stripping of the organic liquid and surfactin accumulation into the receiving phase were less efficient. |
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