Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium |
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Institution: | 1. Departamento de Biocatálisis, ICP-CSIC, Campus UAM-CSIC, 28049 Madrid, Spain;2. Graduate Program in Chemical Engineering (PPGEQ), Laboratory of Enzyme Technologies (LabEnz), Department of Chemical Engineering, Federal University of São Carlos (DEQ/UFSCar), Rod. Washington Luís, km 235, 13565-905 São Carlos, SP, Brazil;3. Department of Biochemistry and Molecular Biology, Faculty of Biology, Complutense University of Madrid, José Antonio Novais 12, Madrid 28040, Spain;4. Center of Excellence in Bionanoscience Research, Member of the External Scientific Advisory Board, King Abdulaziz University, 21589 Jeddah, Saudi Arabia |
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Abstract: | A combination of two lipases was employed to catalyze methanolysis of soybean oil in aqueous medium for biodiesel production. The two lipase genes were cloned from fungal strains Rhizomucor miehei and Penicillium cyclopium, and each expressed successfully in Pichia pastoris. Activities of the 1,3-specific lipase from R. miehei (termed RML) and the non-specific mono- and diacylglycerol lipase from P. cyclopium (termed MDL) were 550 U and 1545 U per ml respectively, and enzymatic properties of these supernatant of fermentation broth (liquid lipase) were stable at 4 °C for >3 months. Under optimized conditions, the ratio of biodiesel conversion after 12 h at 30 °C, using RML alone, was 68.5%. When RML was assisted by addition of MDL, biodiesel conversion ratio was increased to >95% under the same reaction conditions. The results suggested that combination of lipases with different specificity, for enzymatic conversion of more complex lipid substrates, is a potentially useful strategy for biodiesel production. |
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