Improved 2,3-butanediol production from corncob acid hydrolysate by fed-batch fermentation using Klebsiella oxytoca |
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| Institution: | 1. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, PR China;2. Department of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China;1. Research and Development Center, GS Caltex Corporation, Yuseong-gu, Daejeon 305-380, Republic of Korea;2. BioSystem Engineering Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon 305-704, Republic of Korea;1. Hubei Collaborative Innovation Center for Industrial Fermentation, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, PR China;2. Lifecome Bioengineering Institute of Huazhong Agricultural University, the State Key Laboratory of Agricultural Microbiology, College of Life Sciences & Technology, Huazhong Agricultural University, Wuhan 430071, PR China;3. Lifecome Biochemistry Co., Ltd., Pucheng 353400, PR China;1. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China;2. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China;1. Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-District, Muang District, 30000 Nakhon Ratchasima, Thailand;2. Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, Thailand;1. Department of Chemical & Biological Engineering, Korea University, Seoul 02841, Republic of Korea;2. Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea;3. Division of Energy and Environment Technology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea;4. KU-KIST GreenSchool, Graduate School of Energy and Environment, Korea University, Seoul 02841, Republic of Korea;1. Department of Chemical and Biological Engineering, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul, 02841, Republic of Korea;2. Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-Ro, Nowon-Gu, Seoul, 01897, Republic of Korea;3. Department of Biosystem Engineering and Bioenergy, Polytechnic Research Institute of Nepal (PORIN), Koteshwor, Kathmandu, Nepal;4. Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia |
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| Abstract: | Corncob acid hydrolysate, detoxed by sequently boiling, overliming and activated charcoal adsorption, was used for 2,3-butanediol production by Klebsiella oxytoca ACCC 10370. The effects of acetate in hydrolysate and pH on 2,3-butanediol production were investigated. It was found that acetic acid in hydrolysate inhibited the growth of K. oxytoca while benefited the 2,3-butanediol yield. With the increase in acetic acid concentration in medium from 0 to 4 g/l, the lag phase was prolonged and the specific growth rate decreased. The acetic acid inhibition on cell growth can be alleviated by adjusting pH to 6.3 prior to fermentation and a substrate fed-batch strategy with a low initial acetic acid concentration. Under the optimum condition, a maximal 2,3-butanediol concentration of 35.7 g/l was obtained after 60 h of fed-batch fermentation, giving a yield of 0.5 g/g reducing sugar and a productivity of 0.59 g/h l. |
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