Development of the magnetic beads for dye ligand affinity chromatography and application to magnetically stabilized fluidized bed system |
| |
| Affiliation: | 1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada;2. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada;3. Department of Clinical Biochemistry, University Health Network, Toronto, ON, Canada;4. Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada;1. Cleveland Diagnostics, 3615 Superior Avenue, Suite 4407B, Cleveland, OH 44114, USA;2. Department of Molecular Medicine and Byrd Alzheimer''s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA;1. Cancer Gene Therapy Group, University of Helsinki, Helsinki, Finland;2. Molecular Cancer Biology Program, University of Helsinki, Helsinki, Finland;3. Finnish Institute for Molecular Medicine, University of Helsinki, Helsinki, Finland;4. Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, 77030 TX, USA;5. “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Faculty of Pharmacy, Analytical Chemistry Department, Romania;6. Universita degli studi di Firenze, Department of Chemistry Ugo Schiff, 3 Via della Lastruccia, Florence, Italy;7. “Ion Chiricuţă” Cancer Institute, Cluj-Napoca, Romania |
| |
| Abstract: | Magnetic poly(2-hydroxyethylmethacrylate) [mPHEMA] beads were prepared by suspension polymerization of HEMA in the presence of Fe3O4 nano-powder. Cibacron Blue F3GA was covalently immobilized to the mPHEMA beads via nucleophilic substitution reaction between chloride of its triazine ring and hydroxyl groups of HEMA under alkaline conditions. The mPHEMA/Cibacron Blue F3GA beads (100–140 μm in diameter) carrying 68.3 μmol Cibacron Blue F3GA per gram polymer were used for β-casein adsorption studies. Adsorption studies were performed under different conditions in a batch system (i.e., pH, β-casein initial concentration, temperature, and ionic strength) and then in a magnetically stabilized fluidized bed (MSFB) system. The swelling ratio of the mPHEMA was 62.1%. The maximum adsorption capacity for batch system was 20.2% lower as compared to the value obtained in MSFB. The mPHEMA/Cibacron Blue F3GA beads could be repeatedly applied for β-casein adsorption without significant losses in the adsorption capacity. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
