Disruption and eradication of P. aeruginosa biofilms using nitric oxide-releasing chitosan oligosaccharides |
| |
| Authors: | Katelyn P. Reighard David B. Hill Graham A. Dixon Brittany V. Worley |
| |
| Affiliation: | 1. Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA;2. The Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA;3. Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA |
| |
| Abstract: | Biofilm disruption and eradication were investigated as a function of nitric oxide- (NO) releasing chitosan oligosaccharide dose and the results compared with control (ie non-NO-releasing) chitosan oligosaccharides and tobramycin. Quantification of biofilm expansion/contraction and multiple-particle tracking microrheology were used to assess the structural integrity of the biofilm before and after antibacterial treatment. While tobramycin had no effect on the physical properties of the biofilm, NO-releasing chitosan oligosaccharides exhibited dose-dependent behavior with biofilm degradation. Control chitosan oligosaccharides increased biofilm elasticity, indicating that the scaffold may mitigate the biofilm disrupting power of nitric oxide somewhat. The results from this study indicate that nitric oxide-releasing chitosan oligosaccharides act as dual-action therapeutics capable of eradicating and physically disrupting P. aeruginosa biofilms. |
| |
| Keywords: | Nitric oxide P. aeruginosa biofilm rheology |
|
|
