Effect of adsorbed fibronectin on the differential adhesion of osteoblast-like cells and Staphylococcus aureus with and without fibronectin-binding proteins |
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| Authors: | Y. Wang G. Subbiahdoss J. de Vries H.C. van der Mei H.J. Busscher |
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| Affiliation: | 1. Department of Biomedical Engineering , W.J. Kolff Institute, University Medical Center and University of Groningen , The Netherlands;2. Department of Chemical Engineering and Materials Science , Stevens Institute of Technology , Hoboken , NJ , USA;3. Department of Biomedical Engineering , W.J. Kolff Institute, University Medical Center and University of Groningen , The Netherlands |
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| Abstract: | The influence of fibronectin (Fn) coated surfaces patterned with poly(ethylene glycol) microgels having inter-gel spacings between 0.5 and 3.0 μm on the adhesion of Staphylococcus aureus strains with and without Fn-binding proteins and cellular adhesion/spreading was investigated. Quantitative force measurements between a S. aureus cell and a patterned surface showed that the adhesion force between the bacterium and the patterned surface increased substantially after Fn adsorption, regardless of the strain used, but decreased with decreasing inter-gel spacing. In flow-chamber experiments, the Fn-binding strain adhered at a higher rate after Fn adsorption than the strain lacking Fn-binding proteins. In both cases, the adhesion rates decreased with decreasing inter-gel spacing. Osteoblast-like cells could bind to patterned surfaces despite the microgels, and adsorbed Fn substantially amplified this effect. Even under highly non-adhesive conditions associated with closely spaced microgels, adsorbed Fn preserves a window of inter-gel spacing around 1 μm where the adhesion of staphylococcal cells is hindered while cells can still adhere and spread. |
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| Keywords: | hydrogel cell-material interactions patterning, infection PEG bacterial adhesion |
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