Differences in colonisation of five marine bacteria on two types of glass surfaces |
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| Authors: | N Mitik-Dineva J Wang V K Truong P R Stoddart F Malherbe R J Crawford |
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| Institution: | 1. Faculty of Life and Social Sciences , Swinburne University of Technology , PO Box 218, Hawthorn , Victoria , 3122 , Australia;2. IRIS, Swinburne University of Technology , PO Box 218, Hawthorn , Victoria , 3122 , Australia;3. Center for Atom Optics and Ultrafast Spectroscopy, Swinburne University of Technology , PO Box 218, Hawthorn , Victoria , 3122 , Australia |
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| Abstract: | The retention patterns of five taxonomically different marine bacteria after attachment on two types of glass surfaces, as-received and chemically etched, have been investigated. Contact angle measurements, atomic force microscopy (AFM), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), X-ray fluorescence spectroscopy (XRF) and X-ray photoelectron spectrometry (XPS) were employed to investigate the impact of nanometer scale surface roughness on bacterial attachment. Chemical modification of glass surfaces resulted in a ～1 nm decrease in the average surface roughness (R a) and the root-mean-squared roughness (Rq ) and in a ～8 nm decrease in the surface height and the peak-to-peak (R max) and the 10-point average roughness (Rz ). The study revealed amplified bacterial attachment on the chemically etched, nano-smoother glass surfaces. This was a consistent response, notwithstanding the taxonomic affiliation of the selected bacteria. Enhanced bacterial attachment was accompanied by elevated levels of secreted extracellular polymeric substances (EPS). An expected correlation between cell surface wettability and the density of the bacterial attachment on both types of glass surfaces was also reported, while no correlation could be established between cell surface charge and the bacterial retention pattern. |
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| Keywords: | marine bacteria attachment glass surfaces nanometre scale roughness |
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