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Passive Protection Assay of Monoclonal Antibodies Against Dengue Virus in Suckling Mice
Authors:Zongtao Chen  Li-Mei Liu  Na Gao  Xiao-Feng Xu  Jun-Lei Zhang  Jia-Li Wang  Jing An
Institution:(1) Department of Microbiology, Third Military Medical University, 400042 Chongqing, People’s Republic of China;(2) Institute of Pathology, Southwest Hospital, Third Military Medical University, 400042 Chongqing, People’s Republic of China;(3) Department of Microbiology School of Basic Medical Sciences, Capital Medical University, You An Min, 100069 Beijing, People’s Republic of China
Abstract:Dengue fever and dengue hemorrhagic fever/dengue shock syndrome are highly infectious diseases caused by dengue virus (DV). Specific monoclonal antibodies (mAbs) against DV are vital for diagnosis, pathological studies, and passive immune therapy. In this study, purified DV serotype 2 (DV2) was used as antigen and BALB/c mice were immunized to induce specific antibodies. We established five hybridoma cell lines, called 78#, 1E7, 7F7, 8F12, and 8H1, respectively, and evaluated them by enzyme-linked immunosorbent assay, indirect immunofluorescence assay, Western blot, plaque reduction neutralization test, and suckling mice protection assay. Lines 78#, 1E7, 7F7, and 8F12 showed a neutralizing effect, and lines 78#, 1E7, 8F12, and 8H1 recognized envelope glycoprotein of DV2. Among them, lines 78# and 8F12 had stronger neutralizing ability in vitro and could protect some suckling mice from virus challenge. Our results demonstrate that immunization with purified virion is efficient for the production of specific neutralizing mAbs against DV2, and these mAbs could be useful tools for studying or treating DV infection.
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