The in vivo equivalence of a cell-free system for RNA processing and transport |
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Authors: | D E Schumm T E Webb |
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Institution: | Department of Physiological Chemistry, Ohio State University College of medicine, Columbus, Ohio 43210 USA |
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Abstract: | The equivalence of messenger RNA released (transported) from isolated rat liver nuclei to three selected media, with messenger RNA normally released to liver cytoplasm , has been evaluated by competitive DNA: RNA hybridization. Near normal nuclear restriction was exhibited by nuclei in media fortified with ATP, salts, spermidine and dialyzed cytosol. The RNA transport in the latter system was markedly inhibited by colchicine as was also the transport of RNA . Both nuclear restriction and sensitivity of the RNA transport to colchicine in media lacking spermidine and cytosol deviated significantly from the norm. The results emphasize the importance of establishing the equivalence in cell-free systems designed to study RNA synthesis, processing and transport. |
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