Ethanol-induced pseudohyphal transition in the cells of Candida tropicalis: participation of phosphoinositide signal transduction |
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Authors: | Suzuki Takahito Omi Kazuo Ito Takuya Takata Toshiya Kikutsugi Hideo Fujimura Takaji Kono Keita Kamihara Teijiro |
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Affiliation: | Department of Biological Sciences, Faculty of Science, Nara Women's University, Nara, Japan. takahito@cc.nara-wu.ac.jp |
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Abstract: | Ethanol-induced pseudohyphal development in the cells of Candida tropicalis Pk233 was accompanied by the transient accumulation of inositol 1,4,5-trisphosphate (IP3) that occurred at an early growth stage. The concomitant addition of myo-inositol prevented the activation of IP3 accumulation and cancelled pseudohyphal development in the presence of ethanol. The addition of a specific phospholipase C inhibitor, U73 122, inhibited ethanol-induced pseudohyphal transition at the concentrations of subinhibitory levels of cell growth. Pseudohyphal development was also induced by the Ca2+ ionophore, A23 187 in the absence of ethanol. The effect of A23 187 on the development of pseudohyphae was little influenced by myo-inositol, but stimulated by concomitant addition of 12-O-tetradecanoylphorbol 13-acetate. These results suggest that ethanol activated phospholipase C in competition with myo-inositol, and the resulting IP3-Ca2+ and protein kinase C pathways of PI signal transduction may work in pseudohyphal transition. |
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Keywords: | Candida tropicalis pseudohyphal transition ethanol inositol PI signal transduction |
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