Serine hydrolases in differentiating Rhodomicrobium vannielii swarmer cells |
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| Authors: | Russell George C. Mann Nicholas H. |
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| Affiliation: | (1) Department of Biological Sciences, University of Warwick, CV4 7AL Coventry, UK |
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| Abstract: | 3H-diisopropylfluorophosphate (DFP) was used to specifically label serine hydrolases in differentiating Rhodomicrobium vannielii swarmer cells. Fluorography of SDS-polyacrylamide gels revealed several changes in the pattern of 3H-DFP-labelled polypeptides, including the appearance of a new species and apparent increases in the amounts of others. Most of these changes occurred late in differentiation and may be associated with cell division events.Abbreviations DFP diisopropylfluorophosphate - SDS sodium dodecyl sulphate |
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| Keywords: | Bacterial differentiation 3H-diisopropylfluorophosphate labelling Serine proteases Gel electrophoresis Rhodomicrobium vannielii Swarmer cells |
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