Development of DNA microarray for pathogen detection |
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| Authors: | Seung?Min?Yoo Ki?Chang?Keum So?Young?Yoo Jun?Yong?Choi Kyung?Hee?Chang Nae?Choon?Yoo Won?Min?Yoo June?Myung?Kim Duke?Lee Email author" target="_blank">Sang?Yup?LeeEmail author |
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| Institution: | 1.Medigenes Co.,Seoul,Korea;2.Department of Chemical & Biomolecular Engineering and BioProcess Engineering Research Center,KAIST,Daejeon,Korea;3.Department of Radiation Oncology,Yonsei University College of Medicine,Seoul,Korea;4.Department of Internal Medicine,Yonsei University College of Medicine,Seoul,Korea;5.Department of Oncology and Cancer Center,Yonsei University College of Medicine,Seoul,Korea;6.Department of Plastic & Reconstructive Surgery,Yonsei University College of Medicine,Seoul,Korea;7.Harvard School of Dental Medicine,Boston,USA;8.Department of Medicine and Pharmacology,Yale University School of Medicine,New Haven,USA;9.CambridgeMED Co.,Cambridge,USA |
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| Abstract: | Pathogens pose a significant threat to humans, animals, and plants. Consequently, a considerable effort has been devoted to
developing rapid, convenient, and accurate assays for the detection of these unfavorable organisms. Recently, DNA-microarray
based technology is receiving much attention as a powerful tool for pathogen detection. After the target gene is first selected
for the unique identification of microorganisms, species-specific probes are designed through bioinformatic analysis of the
sequences, which uses the information present in the databases. DNA samples, which were obtained from reference and/or clinical
isolates, are properly processed and hybridized with species-specific probes that are immobilized on the surface of the microarray
for fluorescent detection. In this study, we review the methods and strategies for the development of DNA microarray for pathogen
detection, with the focus on probe design. |
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