家蚕胚后发育中过氧化氢的代谢及其意义(英文)

活性氧参与生物体内复杂的代谢过程。本文对它在家蚕个体发育过程中的生物学功能进行了初步探讨。 取１岁龄家蚕蛹分别注射Ｈ２Ｏ２、脱皮激素（Ｅｃｄｎ）、保幼激素（ＪＨ）和还原型谷胱苷肽（ＧＳＨ），培养；定期取样测定家蚕体内Ｈ２Ｏ２含量。 对家蚕整个幼虫期（Ｆｉｇ．１），尤其是大眠期（Ｆｉｇ．２）、化蛹期（Ｔａｂｌｅ１）、蛹期（Ｆｉｇ．３－ａ，ｂ，ｃ）以及成虫期（Ｆｉｇ．４， Ｔａｂｌｅ ２）的研究结果表明，家蚕体内Ｈ２Ｏ２代谢具有如下特点：（１）入眠、化蛹、化蛾和死亡前Ｈ２Ｏ２含量都显著下降；（２）幼虫期１－３龄Ｈ２Ｏ２含量逐步下降，４～５龄Ｈ２Ｏ２含量回升，蛹期和成虫期Ｈ２Ｏ２含量与４～５龄接近；（３）每个龄期的中期Ｈ２Ｏ２含量最高；（４）ＣＡＴ活性与Ｈ２Ｏ２含量呈负相关变化，前者迟于后者；另外，ＣＡＴ活性远远大于ＳＯＤ活性；（５）Ｅｃｄｎ、ＧＳＨ处理可以降低家蚕蛹期Ｈ２Ｏ２含量，并使其提前下降，ＪＨ、Ｈ２Ｏ２处理含量下降，并相应地提早推迟化蛾；（６）成虫期雄蛾Ｈ２Ｏ２含量、ＳＯＤ和ＣＡＴ活性都显著高于同时期雌蛾。家蚕体内Ｈ２Ｏ２含量的变化与其发育密切相关。Ｈ２Ｏ２含量下降是变态的信号；家蚕成虫期Ｈ２Ｏ２ 在性别上的

On Metabolism of Hydrogen Peroxid in Postembryo Development of Bombyx Mori

Investigation on metabolism of postembryo development in silkworm, Bombyx mori, was performed. The results show that: (1) The level of H2O2 decreased significantly before later developmental stages, such as molt, pupation and eclosion; (2) The levels of H2O2 and activities of SOD and CAT were the highest in the first stadium and the lowest in the third stadium; (3) Activity of SOD was lower and that of CAT was higher in molting stage; (4) The levels of H2O2 and activities of SOD and CAT in female moth were much higher than those in male moth; (5) The activity of CAT was significantly higher than that of SOD in the same time; (6) Eclosion of pupae with exotic ecdysone (Ecdn) was moved up and that with juvenile hormone (JH) was delayed than those of control, which was accompanied by earlier or later decline of H2O2; (7) The moving up or delayed eclosion of pupae with GSH or H2O2 treatments was accompanied by the earlier or delayed decline of H2O2; (8) there exist complex relations between levels of H2O2 and activities of SOD and CAT.