Quox-1基因同源序列在小鼠精子发生中的表达

Ｑｕｏｘ－１基因是从鹌鹑中分离得到的Ａｎｔｐ类型的一个同源异形盒基因。以＾３２Ｐ标记的Ｑｕｏｘ－１基因的ｃ３片段为探针，采用分子杂交技术确定了小鼠基因组中存在Ｑｕｏｘ－１基因同源序列。以抗ＱＵＯＸ－１蛋白的特异性抗体对幼年小鼠睾丸、成年小鼠睾丸及附睾的蛋白质样品和组织切片，分别进行了Ｗｅｓｔｅｒｎ ｂｌｏｔ分析和免疫组织化学反应。结果证明，性成熟小鼠在精子发生过程中的精子形成阶段有类ＱＵＯＸ－１蛋

EXPRESSION OF A SEQUENCE HOMOLOGOUS TO QUOX-1 GENE DURING MOUSE SPERMATOGENESIS

Quox-1 gene is an Antp type homeobox gene isolated from the quail. By using Southern hybridization analysis with 32p-labeled c3 fragment of Quox-1 cDNA as a probe, we confirmed that there is a sequence homologous to Quox-1 gene in mouse genome and the size of DNA fraqment, after seing digested by EcoRI and hybridized with Quox-1 cDNA probe, was around 2. 3kb. The results of immuno- histochemistry showed that QUoX-1-like protein (the mouse QUOX-1 protein homologue) was specifically expressed in the cells from spermatid to spermatozoon during spermatogenesis of adult mouse. Western blot analysis with QUOX-1 protein antiserum indicated that the molecular weight of QUOX-l-like protein was 40 kD. Probable roles of the sequcence homologous to Quox-1 gene in the regulation of spermeiogenesis and the methodology of immuno- histochemistry for, detecting the expression of homeobox protein were discussed.