| 变应性鼻炎鼻分泌物中细胞内DNA和RNA代谢的观测 |
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| 引用本文： | 沈志忠,陈耀文,林珏龙,王挥戈,王豪,宋德辉.变应性鼻炎鼻分泌物中细胞内DNA和RNA代谢的观测[J].激光生物学报,2002,11(2):129-132. |
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| 作者姓名： | 沈志忠 陈耀文 林珏龙 王挥戈 王豪 宋德辉 |
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| 作者单位： | 1. 汕头大学医学院第一附属医院耳鼻咽喉科,中国广东,汕头,515041
2. 汕头大学医学院中心实验室,中国广东,汕头,515031 |
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| 基金项目： | 广东省医学科学技术研究基金资助项目 (A1999411) |
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| 摘 要： | 本文介绍应变性鼻炎鼻分泌物中细胞内DNA和RNA代谢变化的观测方法。以鼻分泌物中的多种炎性细胞为实验标本，经细胞贴壁、0.1%吖橙染色标记，37℃孵育15min；采用激光扫描共聚焦显微镜对细胞内DNA，RNA形态和含量进行观测。结果表明：本方法应用于变应性鼻炎鼻分泌物细胞内DNA、RNA代谢的研究是切实可行的。
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| 关 键 词： | 变应性鼻炎 鼻分泌物 RNA代谢 观测 炎性细胞 DNA代谢 |
| 文章编号： | 1007-7146(2002)02-0129-04 |
| 收稿时间： | 2001/10/29 |
| 修稿时间： | 2001年10月29 |
Study on the Observation of Intercellular DNA and RNA in Nasal Secretions of the Patients with Allergic Rhinitis |
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| SHEN Zhi-zhong ,CHEN Yao-wen ,LIN Jue-long ,WANG Hui-ge ,WANG Hao ,SONG De-hui.Study on the Observation of Intercellular DNA and RNA in Nasal Secretions of the Patients with Allergic Rhinitis[J].ACTA Laser Biology Sinica,2002,11(2):129-132. |
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| Authors: | SHEN Zhi-zhong CHEN Yao-wen LIN Jue-long WANG Hui-ge WANG Hao SONG De-hui |
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| Institution: | SHEN Zhi-zhong 1,CHEN Yao-wen 2,LIN Jue-long 2,WANG Hui-ge 1,WANG Hao 1,SONG De-hui 1 |
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| Abstract: | The observation of intercellular DNA,RNA in nasal secretions of the patients with allergic rhinitis were recommended.The inflammatory cells in the nasal secretions were fixed,and incubated with 0.1% AO at 37℃ for 15 minutes. The variation of intercellular RNA and RNA/DNA radio of the eosinophils,neutrocyte and lymphocyte in the nasal secretions were observed and measured under the laser scanning confocal microscopy(LSCM). The result showed that it is successful to study the intercellular RNA,DNA metabolize of the inflammatory cells in nasal secretions of the patients with allergic rhinitis. |
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| Keywords: | laser scanning confocal microscopy inflammatiory cell DNA RNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
